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Plos One : Genomic Analysis of Pseudomonas Putidaphage Tf with Localized Single-strand Dna Interruptions, Volume 7

By Poteete, Anthony, R.

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Book Id: WPLBN0003938169
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Genomic Analysis of Pseudomonas Putidaphage Tf with Localized Single-strand Dna Interruptions, Volume 7  
Author: Poteete, Anthony, R.
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
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Publisher: Plos

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Poteete, A. R. (n.d.). Plos One : Genomic Analysis of Pseudomonas Putidaphage Tf with Localized Single-strand Dna Interruptions, Volume 7. Retrieved from http://read.schoollibrary.com/


Description
Description : The complete sequence of the 46,267 bp genome of the lytic bacteriophage tf specific to Pseudomonas putida PpG1 has been determined. The phage genome has two sets of convergently transcribed genes and 186 bp long direct terminal repeats. The overall genomic architecture of the tf phage is similar to that of the previously described Pseudomonas aeruginosa phages PaP3, LUZ24 and phiMR299-2, and 39 out of the 72 products of predicted tf open reading frames have orthologs in these phages. Accordingly, tf was classified as belonging to the LUZ24-like bacteriophage group. However, taking into account very low homology levels between tf DNA and that of the other phages, tf should be considered as an evolutionary divergent member of the group. Two distinguishing features not reported for other members of the group were found in the tf genome. Firstly, a unique end structure – a blunt right end and a 4-nucleotide 39-protruding left end – was observed. Secondly, 14 single-chain interruptions (nicks) were found in the top strand of the tf DNA. All nicks were mapped within a consensus sequence 59-TACT/RTGMC-39. Two nicks were analyzed in detail and were shown to be present in more than 90% of the phage population. Although localized nicks were previously found only in the DNA of T5-like and phiKMV-like phages, it seems increasingly likely that this enigmatic structural feature is common to various other bacteriophages.

 
 



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